Lab 5 Diffusion and Osmosis Objectives a.
Laboratory Exercise Manual Pencil There were three mini-lab procedures carried out during this lab. The first lab exercise was observing animal cells, in this case, my cheek cells. The second lab exercise was observing plant cells, in this case, onion epidermis.
The third lab exercise was observing chloroplasts and biological crystals, in this case, a thin section from the Zebrina plant.
|2 Applications and skills||Jump to navigation Jump to search Osmosis is defined as the diffusion of water solvent from areas of high solvent more amount of water concentration to areas of low solvent less amount of water concentration across a semi-permeable membrane.|
|Related Content||Compare the location of chloroplasts in normal and plasmolyzed cells. What was the cause for the change in the location of the chloroplasts in the two solutions?|
The first thing that was done in this lab exercise was gather materials. I worked with two other classmates that sat at my table. Observation of animal cells squamous epithelium of a cheek Using a toothpick, I carefully scarped the inside of my cheek to get the cells.
I then spread it across the slide, added the Methylene Blue solution and then covered the slide with a cover slip. I placed the slide in the center of the stage and made sure it was secured with the stage clip. The objective lens was already at 10X magnification, so I switched it to 40X magnification.
I moved the stage closer up using the Coarse Adjustment. I also adjusted the lighting of the microscope using the diaphragm. I then switched the magnification to 40X.
I adjusted the Fine Adjustment to get a sharper image of the cell. I was able to see the cheek cell correctly.
you are here->home->Biotechnology and Biomedical Engineering->Cell biology Virtual Lab I->Isolation of Chloroplast.. Isolation of Chloroplast.. Theory. Procedure. Self Evaluation. Animation. Simulator. Assignment. Reference. Feedback. Objective. To isolate chloroplast and estimate the chlorophyll concentration from spinach leaves. Theory. Feb 21, · Osmolarity/molar concentration of potato core cells?? HELPP PLEASE (AP BIOLOGY)? (osmolarity) of the potato core cells" i dont understand!! maybe i should have been paying more attention in class T_T. 1 following. 3 answers 3. Report Abuse. Are you sure you want to delete this answer? Yes feelthefish.com: Resolved. Biology LAB. Lab 2 Carbs, Lipids, and Proteins Lipids, and Proteins. Lab 3 & 4 Cell Types and Histology. Lab 5 Diffusion and Osmosis. Lab 6 Protein Quantification. Lab 7 Enzymes. Lab 8 Brain. Lab 9 Contraction of Glycerinated Muscle with ATP. Determine the Concentration of Water in Living Plant Cells. Table 3. Change in Weight.
I was able to see the Cytoplasm, Nucleus, and the Cell Membrane. Observation of plant cells onion epidermis For this observation, a plant cell was to be seen. An onion bulb was retrieved. Using the forceps, I removed a small slice of the onion and carefully and quickly put it on the slide.
I also added water to ensure that the onion slice would not dry out. I adjusted the lighting again using the diaphragm, to contrast the compartments of the cell.
I moved the stage closer up using the Coarse Adjustment and switched the magnification to 10X. Observation of chloroplast and biological crystals For this observation, I looked at a small section from the Zebrina stem. The stem was gotten from the bucket in front of the classroom.
The small section was obtained by slicing a tiny amount of the stem using the razor blade. It was placed on the slide, followed by the water. When first observed, nothing clear could be seen. It appeared to be that the Zebrina stem was cut too thick.
The stem had to be cut once more, and this time much thinner, but not too thin. The same procedure was repeated again, adding water, placing the cover slip, putting the slide on the stage, adjusting the stage, and making the image sharper.
The second sample proved to be much better. The magnification was already positioned at 10X magnification, which made the cell much clearer to see. Discussion Calculate the resolving power of the lenses on your microscope.
To find the resolving power for each of the lenses on the compound microscope, I used the Abbey equation. I repeated this equation for each magnification, getting the resolving power for each of the lens.In this section, we examine two types of transport phenomena that, at first glance, may seem unrelated: the regulation of cell volume in both plant and animal cells, and the bulk flow of water (the movement of water containing dissolved solutes) across one or more layers of cells.
To maintain their normal cytosolic osmolarity and hence cell. A STUDY OF OSMOSIS. The cytoplasm and/or vacuole of the cell shrinks. In an animal cell, the entire cell shrivels. In a plant cell, the cytoplasm shrinks away from the cell wall. A cell placed in a How quickly and how effectively do these drinks actually rehydrate your body's cells?
Perhaps this lab can help you answer that question. Pre-Lab Questions: Microscope Lab: Estimating Size and Calculating Magnification 1. Draw a sketch of the microscope in your lab notebook. Microscope Lab: Estimating Size and Calculating Magnification IB Internal Assessment of..
DCP and CE Part 1: Estimating Size of Specimens under the Microscope Purpose Fifteen plant cells stretch. you are here->home->Biotechnology and Biomedical Engineering->Cell biology Virtual Lab I->Isolation of Chloroplast..
Isolation of Chloroplast.. Theory. Procedure. Self Evaluation. Animation. Simulator.
Assignment. Reference. Feedback. Objective. To isolate chloroplast and estimate the chlorophyll concentration from spinach leaves. Theory. Plant and animal cells have different characteristic sizes, but they are always really big when compared to bacteria, the microscope we will use in today’s lab is called light or compound microscope and allows.
Estimating the Osmolarity of Plant Cells by Change in Weight Foreword: Osmosis is a type of diffusion, the diffusion of water through a semi-permeable membrane, from an area of low solute concentration to an area of high solute concentration. For example, if a.